RAB11-FIP2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19705R-Cy3 |
| Product Name | RAB11-FIP2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken, Cat, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAB11-FIP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22841 |
| Swiss Prot | Q7L804 |
| Subcellular location | Cell membrane |
| Synonyms | RAB11FIP2;KIAA0941; NRip11; RAB11 family interacting protein 2 (class I); Rab11 family-interacting protein 2; RAB11 FIP2 long isoform; Rab11-FIP2; RFIP2; RFIP2_HUMAN. |
| Background | A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RAB11-FIP2 Polyclonal Antibody, Cy3 Conjugated (bs-19705R-Cy3) validated for, and at what dilution?
bs-19705R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAB11-FIP2 Polyclonal Antibody, Cy3 Conjugated (bs-19705R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken, Cat, Rabbit, GuineaPig.
How should bs-19705R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAB11-FIP2 Polyclonal Antibody, Cy3 Conjugated (bs-19705R-Cy3)?
KLH conjugated synthetic peptide derived from human RAB11-FIP2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-19705R-Cy3)?
Cy3 on bs-19705R-Cy3: excitation / emission 512,550nm/570,615nm.