RAD51D Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19726R-BF647 |
| Product Name | RAD51D Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Cow |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAD51D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5892 |
| Swiss Prot | O75771 |
| Subcellular location | Nucleus |
| Synonyms | DNA repair protein RAD51 homolog 4; RAD51D; R51H3; RAD51 homolog D RAD51-like protein 3; TRAD; RAD51D; RAD51L3 |
| Background | Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RAD51D Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19726R-BF647) validated for, and at what dilution?
bs-19726R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAD51D Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19726R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow.
How should bs-19726R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAD51D Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19726R-BF647)?
KLH conjugated synthetic peptide derived from human RAD51D
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-19726R-BF647)?
AbBy Fluor-647 on bs-19726R-BF647: excitation / emission 650nm/665nm.