RASGRP4 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19731R-BF647-100uL |
| Product Name | RASGRP4 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(ICC)
IF
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RASGRP4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 115727 |
| Swiss Prot | Q8TDF6 |
| Synonyms | RASGRP-4; GRP4_HUMAN; Guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4; RAS guanyl-releasing protein 4; RASGRP 4. |
| Background | The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009] |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is RASGRP4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19731R-BF647) validated for, and at what dilution?
bs-19731R-BF647 is validated for IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RASGRP4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19731R-BF647) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Horse.
How should bs-19731R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RASGRP4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19731R-BF647)?
KLH conjugated synthetic peptide derived from human RASGRP4
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-19731R-BF647)?
AbBy Fluor-647 on bs-19731R-BF647: excitation / emission 650nm/665nm.