phospho-NFATC3 (Ser165) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19787R-BF488 |
| Product Name | phospho-NFATC3 (Ser165) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human NFAT4 around the phosphorylation site of Ser165 |
| Modification Site | Ser165 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4775 |
| Swiss Prot | Q12968 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NFAT4 (phospho S165); p-NFAT4 (phospho S165); C80703; cytoplasmic 3; D8Ertd281e; NF AT4; NF ATc3; NF-AT4; NF-ATc3; NFAC3_HUMAN; NFATc3; NFATx; Nuclear factor of activated T cells, cytoplasmic 3; nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3; Nuclear factor of activated T-cells; T cell transcription factor NFAT4; T-cell transcription factor NFAT4. |
| Background | The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-NFATC3 (Ser165) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-19787R-BF488) validated for, and at what dilution?
bs-19787R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-NFATC3 (Ser165) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-19787R-BF488) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Horse, Chicken, Rabbit.
How should bs-19787R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-NFATC3 (Ser165) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-19787R-BF488)?
KLH conjugated synthesised phosphopeptide derived from human NFAT4 around the phosphorylation site of Ser165
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-19787R-BF488)?
AbBy Fluor-488 on bs-19787R-BF488: excitation / emission 499nm/519nm.