REV3L/Polymerase DNA zeta Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19840R-BF647 |
| Product Name | REV3L/Polymerase DNA zeta Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human REV3L |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5980 |
| Swiss Prot | O60673 |
| Subcellular location | Nucleus |
| Synonyms | POLZ; REV3; DNA polymerase zeta catalytic subunit; Protein reversionless 3-like; REV3-like; hREV3; REV3L |
| Background | Interacts with MAD2L2 to form the error prone DNA polymerase zeta involved in translesion DNA synthesis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is REV3L/Polymerase DNA zeta Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19840R-BF647) validated for, and at what dilution?
bs-19840R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does REV3L/Polymerase DNA zeta Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19840R-BF647) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-19840R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise REV3L/Polymerase DNA zeta Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19840R-BF647)?
KLH conjugated synthetic peptide derived from human REV3L
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-19840R-BF647)?
AbBy Fluor-647 on bs-19840R-BF647: excitation / emission 650nm/665nm.