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phospho-Parvin alpha (Ser8) Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Cow
  • Rabbit
Overview
Catalog # bs-19894R-Cy5-100uL
Product Name phospho-Parvin alpha (Ser8) Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Cow, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthesised phosphopeptide derived from human Parvin alpha around the phosphorylation site of Ser8
Modification Site Ser8
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55742
Swiss Prot Q9NVD7
Subcellular location Cytoplasm, Cell membrane
Synonyms Parvin alpha (phospho S8); p-Parvin alpha (phospho S8); Actopaxin; Alpha parvin; Alpha-parvin; Calponin like integrin linked kinase binding protein; Calponin-like integrin-linked kinase-binding protein; CH ILKBP; CH-ILKBP; FLJ10793; FLJ12254; Matrix remodelling associated 2; Matrix remodelling associated protein 2; Matrix-remodeling-associated protein 2; MXRA 2; MXRA2; PARV A; PARVA; PARVA_HUMAN.
Background This gene encodes a member of the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion, motility and survival. [provided by RefSeq, Dec 2010]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is phospho-Parvin alpha (Ser8) Polyclonal Antibody, Cy5 Conjugated (bs-19894R-Cy5) validated for, and at what dilution?

bs-19894R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-Parvin alpha (Ser8) Polyclonal Antibody, Cy5 Conjugated (bs-19894R-Cy5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Rabbit.

How should bs-19894R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-Parvin alpha (Ser8) Polyclonal Antibody, Cy5 Conjugated (bs-19894R-Cy5)?

KLH conjugated synthesised phosphopeptide derived from human Parvin alpha around the phosphorylation site of Ser8

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-19894R-Cy5)?

Cy5 on bs-19894R-Cy5: excitation / emission 625,650nm/670nm.