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PPP2R5E Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Horse
Overview
Catalog # bs-19971R-BF750
Product Name PPP2R5E Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Horse
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PPP2R5E
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5529
Swiss Prot Q16537
Subcellular location Cytoplasm
Synonyms Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform; PPP2R5E; PP2A B subunit isoform B'-epsilon; PP2A B subunit isoform B56-epsilon; PP2A B subunit isoform PR61-epsilon; PP2A B subunit isoform R5-epsilon; PPP2R5E
Background The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PPP2R5E Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19971R-BF750) validated for, and at what dilution?

bs-19971R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PPP2R5E Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19971R-BF750) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Horse.

How should bs-19971R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PPP2R5E Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19971R-BF750)?

KLH conjugated synthetic peptide derived from human PPP2R5E

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-19971R-BF750)?

AbBy Fluor-750 on bs-19971R-BF750: excitation / emission 749nm/775nm.