PACE4 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PACE4 Polyclonal Antibody, Unconjugated (bs-19997R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PACE4 Polyclonal Antibody, Unconjugated (bs-19997R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Lane 1: Rat Testis tissue lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human SH-SY5Y cell lysates probed with PACE4 Polyclonal Antibody, Unconjugated (bs-19997R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Rat Testis tissue lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human SH-SY5Y cell lysates probed with PACE4 Polyclonal Antibody, Unconjugated (bs-19997R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-19997R
Product Name	PACE4 Polyclonal Antibody
Applications	WB ELISA
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PACE4
Immunogen Range	201-300/969
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5046
Swiss Prot	P29122
Synonyms	Paired basic amino acid cleaving enzyme 4; paired basic amino acid cleaving system 4; PCSK6; PCSK6_HUMAN; Proprotein convertase subtilisin/kexin type 6; SPC4; Subtilisin like protease; Subtilisin-like proprotein convertase 4; subtilisin/kexin like protease PACE4; Subtilisin/kexin-like protease PACE4.
Background	Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Application Dilution
WB
ELISA