SIGLEC11 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20001R-BF680 |
| Product Name | SIGLEC11 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SIGLEC11 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 114132 |
| Swiss Prot | Q96RL6 |
| Subcellular location | Cell membrane |
| Synonyms | sialic acid binding Ig like lectin 11; sialic acid binding lectin 11; Siglec 11; SIG11_HUMAN. |
| Background | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,8-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SIGLEC11 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-20001R-BF680) validated for, and at what dilution?
bs-20001R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SIGLEC11 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-20001R-BF680) react with?
Tested reactivity: Human.
How should bs-20001R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SIGLEC11 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-20001R-BF680)?
KLH conjugated synthetic peptide derived from human SIGLEC11
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-20001R-BF680)?
AbBy Fluor-680 on bs-20001R-BF680: excitation / emission 679nm/702nm.