EGF Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2009R-Cy7 |
| Product Name | EGF Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse EGF |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 13645 |
| Swiss Prot | P01132 |
| Subcellular location | Cell membrane |
| Synonyms | AI790464; Pro-epidermal growth factor; EGF |
| Background | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EGF Polyclonal Antibody, Cy7 Conjugated (bs-2009R-Cy7) validated for, and at what dilution?
bs-2009R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EGF Polyclonal Antibody, Cy7 Conjugated (bs-2009R-Cy7) react with?
Tested reactivity: Mouse.
How should bs-2009R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EGF Polyclonal Antibody, Cy7 Conjugated (bs-2009R-Cy7)?
KLH conjugated synthetic peptide derived from mouse EGF
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-2009R-Cy7)?
Cy7 on bs-2009R-Cy7: excitation / emission 743nm/767nm.