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EGF Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
Overview
Catalog # bs-2009R-PE-100uL
Product Name EGF Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse EGF
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 13645
Swiss Prot P01132
Subcellular location Cell membrane
Synonyms AI790464; Pro-epidermal growth factor; EGF
Background EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is EGF Polyclonal Antibody, PE Conjugated (bs-2009R-PE) validated for, and at what dilution?

bs-2009R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EGF Polyclonal Antibody, PE Conjugated (bs-2009R-PE) react with?

Tested reactivity: Mouse.

How should bs-2009R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EGF Polyclonal Antibody, PE Conjugated (bs-2009R-PE)?

KLH conjugated synthetic peptide derived from mouse EGF

What are the excitation and emission wavelengths of the PE conjugate (bs-2009R-PE)?

PE on bs-2009R-PE: excitation / emission 496,564nm/578nm.