Angiotensin III Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20101R-BF488 |
| Product Name | Angiotensin III Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | The immunogen used generate this antibody is RVYIHPF, corresponding to amino acids 35-41 of human Angiotensinogen. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Horse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide of human Angiotensin III |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 183 |
| Subcellular location | Secreted |
| Synonyms | Alpha 1 antiproteinase, antitrypsin; Ang II; ANG III; Angiotensin I; Angiotensin II; Angiotensin III; Angiotensinogen; Angiotensinogen serpin peptidase inhibitor, clade A member 8; ANHU; Pre angiotensinogen; Serine or cysteine proteinase inhibitor; Serpin A8; SERPINA8; AT-2; AT-II; ANGT_HUMAN. |
| Background | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Angiotensin-3: stimulates aldosterone release. Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Angiotensin III Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20101R-BF488) validated for, and at what dilution?
bs-20101R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Angiotensin III Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20101R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse.
How should bs-20101R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Angiotensin III Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20101R-BF488)?
KLH conjugated synthetic peptide of human Angiotensin III
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-20101R-BF488)?
AbBy Fluor-488 on bs-20101R-BF488: excitation / emission 499nm/519nm.