EGF Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2010R-RBITC-100uL |
| Product Name | EGF Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat EGF |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 25313 |
| Swiss Prot | P07522 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | Pro-epidermal growth factor; EGF |
| Background | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EGF Polyclonal Antibody, RBITC Conjugated (bs-2010R-RBITC) validated for, and at what dilution?
bs-2010R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EGF Polyclonal Antibody, RBITC Conjugated (bs-2010R-RBITC) react with?
Tested reactivity: Rat.
How should bs-2010R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EGF Polyclonal Antibody, RBITC Conjugated (bs-2010R-RBITC)?
KLH conjugated synthetic peptide derived from rat EGF
What are the excitation and emission wavelengths of the RBITC conjugate (bs-2010R-RBITC)?
RBITC on bs-2010R-RBITC: excitation / emission 540nm/625nm.