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EGF Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB

Reactivity

  • Rat
Overview
Catalog # bs-2010R-PE-Cy5-100uL
Product Name EGF Polyclonal Antibody, PE-Cy5 Conjugated
Applications
WB
Reactivity Rat
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat EGF
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 25313
Swiss Prot P07522
Subcellular location Secreted, Cell membrane
Synonyms Pro-epidermal growth factor; EGF
Background EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is EGF Polyclonal Antibody, PE-Cy5 Conjugated (bs-2010R-PE-Cy5) validated for, and at what dilution?

bs-2010R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EGF Polyclonal Antibody, PE-Cy5 Conjugated (bs-2010R-PE-Cy5) react with?

Tested reactivity: Rat.

How should bs-2010R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EGF Polyclonal Antibody, PE-Cy5 Conjugated (bs-2010R-PE-Cy5)?

KLH conjugated synthetic peptide derived from rat EGF

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-2010R-PE-Cy5)?

PE-Cy5 on bs-2010R-PE-Cy5: excitation / emission 488nm/670nm.