NIT2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti- NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Anti- NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse embryo tissue labeled Anti-NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse embryo tissue labeled Anti-NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

A431 cell lysates probed with NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

A431 cell lysates probed with NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-2012R
Product Name	NIT2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat NIT2
Immunogen Range	41-140/267
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	288174
Swiss Prot	Q497B0
Subcellular location	Cytoplasm
Synonyms	RGD1310494; Omega-amidase NIT2; Nitrilase homolog 2; Nit2
Background	Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

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