FAIM3 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20130R-Cy3 |
| Product Name | FAIM3 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FAIM3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9214 |
| Swiss Prot | O60667 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | TOSO; FAIM3; Fas apoptotic inhibitory molecule 3; Regulator of Fas-induced apoptosis Toso |
| Background | May play a role in the immune system processes. Protects cells from FAS-, TNF alpha- and FADD-induced apoptosis without increasing expression of the inhibitors of apoptosis BCL2 and BCLXL. Seems to activate an inhibitory pathway that prevents CASP8 activation following FAS stimulation, rather than blocking apoptotic signals downstream. May inhibit FAS-induced apoptosis by preventing CASP8 processing through CFLAR up-regulation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FAIM3 Polyclonal Antibody, Cy3 Conjugated (bs-20130R-Cy3) validated for, and at what dilution?
bs-20130R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FAIM3 Polyclonal Antibody, Cy3 Conjugated (bs-20130R-Cy3) react with?
Tested reactivity: Human.
How should bs-20130R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FAIM3 Polyclonal Antibody, Cy3 Conjugated (bs-20130R-Cy3)?
KLH conjugated synthetic peptide derived from human FAIM3
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-20130R-Cy3)?
Cy3 on bs-20130R-Cy3: excitation / emission 512,550nm/570,615nm.