HSV tag Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20157R-BF647-100uL |
| Product Name | HSV tag Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide HSV tag |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | HSV epitope tag; QPELAPEDPED tag; Envelope glycoprotein D (Human herpesvirus 1); Envelope glycoprotein D; US6 (Human herpesvirus 1); gD. |
| Background | The HSV (herpes simplex virus) epitope tag is frequently engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized HSV peptide epitope represents the amino acid sequence QPELAPEDPED. HSV Tag antibody can recognize C-terminal, internal, and N-terminal HSV-tagged proteins. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HSV tag Antibody, AbBy Fluor-647 Conjugated (bs-20157R-BF647) validated for, and at what dilution?
bs-20157R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSV tag Antibody, AbBy Fluor-647 Conjugated (bs-20157R-BF647) react with?
Tested reactivity: Species independent.
How should bs-20157R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSV tag Antibody, AbBy Fluor-647 Conjugated (bs-20157R-BF647)?
KLH conjugated synthetic peptide HSV tag
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-20157R-BF647)?
AbBy Fluor-647 on bs-20157R-BF647: excitation / emission 650nm/665nm.