EYA1 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20158R-Cy7 |
| Product Name | EYA1 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EYA1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2138 |
| Swiss Prot | Q99502 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | BOP; BOR; BOS1; OFC1; Eyes absent homolog 1; EYA1 |
| Background | Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears. Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EYA1 Polyclonal Antibody, Cy7 Conjugated (bs-20158R-Cy7) validated for, and at what dilution?
bs-20158R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EYA1 Polyclonal Antibody, Cy7 Conjugated (bs-20158R-Cy7) react with?
Tested reactivity: Human.
How should bs-20158R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EYA1 Polyclonal Antibody, Cy7 Conjugated (bs-20158R-Cy7)?
KLH conjugated synthetic peptide derived from human EYA1
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-20158R-Cy7)?
Cy7 on bs-20158R-Cy7: excitation / emission 743nm/767nm.