HIP55 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20165R-BF488 |
| Product Name | HIP55 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIP55 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 28988 |
| Swiss Prot | Q9UJU6 |
| Subcellular location | Cytoplasm |
| Synonyms | ABP1; Actin-binding protein 1; Cervical mucin associated protein; Cervical mucin-associated protein; Cervical SH3P7; CMAP; Dbnl; DBNL_HUMAN; Drebrin F; drebrin like; Drebrin like protein; Drebrin-F; Drebrin-like protein; HIP 55; HIP-55; HPK1 interacting protein of 55 kDa; HPK1-interacting protein of 55 kDa; SH3 domain containing protein 7; SH3 domain-containing protein 7; SH3P7; Src Homology 3 Domain Containing Protein; src homology 3 domain containing protein HIP |
| Background | Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HIP55 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20165R-BF488) validated for, and at what dilution?
bs-20165R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIP55 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20165R-BF488) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-20165R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIP55 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20165R-BF488)?
KLH conjugated synthetic peptide derived from human HIP55
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-20165R-BF488)?
AbBy Fluor-488 on bs-20165R-BF488: excitation / emission 499nm/519nm.