Integrin Alpha V + Beta 1 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2016R-PE |
| Product Name | Integrin Alpha V + Beta 1 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | Mixed KLH conjugated syntheticpeptides for human Integrin Alpha V and human Integrin Beta1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3685, 3688 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | Integrin alpha-V: Integrin alpha-V, Vitronectin receptor subunit alpha, CD51 Integrin beta-1: Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29 |
| Background | The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | |
Frequently asked questions
Which applications is Integrin Alpha V + Beta 1 Polyclonal Antibody, PE Conjugated (bs-2016R-PE) validated for, and at what dilution?
bs-2016R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Integrin Alpha V + Beta 1 Polyclonal Antibody, PE Conjugated (bs-2016R-PE) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse.
How should bs-2016R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Integrin Alpha V + Beta 1 Polyclonal Antibody, PE Conjugated (bs-2016R-PE)?
Mixed KLH conjugated syntheticpeptides for human Integrin Alpha V and human Integrin Beta1
What are the excitation and emission wavelengths of the PE conjugate (bs-2016R-PE)?
PE on bs-2016R-PE: excitation / emission 496,564nm/578nm.