STAT2 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20176R-BF405 |
| Product Name | STAT2 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Horse |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human STAT2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6773 |
| Swiss Prot | P52630 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | P113; ISGF-3; STAT113; Signal transducer and activator of transcription 2; STAT2 |
| Background | Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is STAT2 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-20176R-BF405) validated for, and at what dilution?
bs-20176R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does STAT2 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-20176R-BF405) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Horse.
How should bs-20176R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise STAT2 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-20176R-BF405)?
KLH conjugated synthetic peptide derived from human STAT2
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-20176R-BF405)?
AbBy Fluor-405 on bs-20176R-BF405: excitation / emission 401nm/421nm.