MCU Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MCU Polyclonal Antibody, Unconjugated (bs-20189R) at 1:500 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MCU Polyclonal Antibody, Unconjugated (bs-20189R) at 1:500 overnight at 4\u00b0C, DAB staining.

$25 ug total protein per lane of various lysates (see on figure) probed with MCU polyclonal antibody, unconjugated (bs-20189R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with MCU polyclonal antibody, unconjugated (bs-20189R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ELISA
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-20189R
Product Name	MCU Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MCU
Immunogen Range	300-351/351
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	90550
Swiss Prot	Q8NE86
Synonyms	MCU_HUMAN; C10orf42; Ccdc109a; Coiled-coil domain-containing protein 109A; Mitochondrial Calcium Uniporter.
Background	This gene encodes a calcium transporter that localizes to the mitochondrial inner membrane. The encoded protein interacts with mitochondrial calcium uptake 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
Application Dilution
WB	IHC-P1:200-400
ELISA	IHC-F1:100-500
IHC-P	IFICC1:50-200
IHC-F	IF
IF
ICC/IF