Phospho-CDK9 (Thr29) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20205R-BF750 |
| Product Name | Phospho-CDK9 (Thr29) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Chicken, Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human CDK9 around the phosphorylation site of Thr29 |
| Modification Site | Thr29 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1025 |
| Swiss Prot | P50750 |
| Subcellular location | Nucleus |
| Synonyms | CDK9_HUMAN; Cyclin-dependent kinase 9; EC:2.7.11.22; EC:2.7.11.23; CDK9; CDC2L4; TAK; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit; |
| Background | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. |
| Application Dilution | |
| IF | |
Frequently asked questions
Which applications is Phospho-CDK9 (Thr29) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-20205R-BF750) validated for, and at what dilution?
bs-20205R-BF750 is validated for IF.
Which species does Phospho-CDK9 (Thr29) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-20205R-BF750) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Zebrafish.
How should bs-20205R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CDK9 (Thr29) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-20205R-BF750)?
KLH conjugated Synthesised phosphopeptide derived from human CDK9 around the phosphorylation site of Thr29