phospho-KLF5 (Ser275) Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-phospho-KLF5(Ser275)Polyclonal Antibody, Unconjugated (bs-2020R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti-phospho-KLF5(Ser275) Polyclonal Antibody, Unconjugated (bs-2020R) followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human HT-1080 cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human A431 cell lysates probed with phospho-KLF5 (Ser275) Polyclonal Antibody, Unconjugated (bs-2020R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human HT-1080 cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human A431 cell lysates probed with phospho-KLF5 (Ser275) Polyclonal Antibody, Unconjugated (bs-2020R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-2020R
Product Name	phospho-KLF5 (Ser275) Polyclonal Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthesised phosphopeptide derived from human KLF5 around the phosphorylation site of Ser275
Modification Site	Ser275
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	688
Swiss Prot	Q13887
Subcellular location	Nucleus
Synonyms	KLF5 (phospho Ser275); KLF5 (phospho S275); Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2;BTEB2; intestine enriched krppel-like factor; IKLF; Kruppel like factor 5?Transcription factor BTEB 2; Transcription factor BTEB2; KLF5_HUMAN.
Application Dilution
WB	=1:500-2000
IHC-P
IHC-F
IF