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Musashi 1 Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-20241r-rbitc
Product Name Musashi 1 Polyclonal Antibody, RBITC Conjugated
Applications
IF(ICC) IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Musashi 1
Immunogen Range 151-250/362
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4440
Swiss Prot O43347
Synonyms Musashi 1 / Msi1; Msi 1; Msi1; Msi-1; MSI1H_HUMAN; Musashi homolog 1; Musashi-1; Musashi1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1.
Background Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.
Application Dilution
IF(ICC) 1:50-200
IF

Frequently asked questions

Which applications is Musashi 1 Polyclonal Antibody, RBITC Conjugated (bs-20241R-RBITC) validated for, and at what dilution?

bs-20241R-RBITC is validated for IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Musashi 1 Polyclonal Antibody, RBITC Conjugated (bs-20241R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-20241R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Musashi 1 Polyclonal Antibody, RBITC Conjugated (bs-20241R-RBITC)?

KLH conjugated synthetic peptide derived from human Musashi 1 Immunogen range: 151-250/362.

What are the excitation and emission wavelengths of the RBITC conjugate (bs-20241R-RBITC)?

RBITC on bs-20241R-RBITC: excitation / emission 570nm/595nm.