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CYP2C9 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CYP2C9 Polyclonal Antibody, Unconjugated (bs-20243R) at 1:500 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CYP2C9 Polyclonal Antibody, Unconjugated (bs-20243R) at 1:500 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates probed with CYP2C9 Polyclonal Antibody, Unconjugated (bs-20243R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates probed with CYP2C9 Polyclonal Antibody, Unconjugated (bs-20243R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-20243r
Product Name CYP2C9 Polyclonal Antibody
Applications
ELISA IHC-P IHC-F IF ICC/IF
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CYP2C9
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1559
Swiss Prot P11712
Subcellular location Cytoplasm
Synonyms Cytochrome P450 2C9; The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme CPC9; CYP2C; CYP2C10; CYP2C9; Cytochrome p4502C9; P450 MP; P450 PB 1; P450 PB1; P450IIC9; P450MP; S mephenytoin 4 hydroxylase; CP2C9_HUMAN.
Application Dilution
ELISA =1:5000-10000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500
ICC/IF ICC/IF=1:100-500

Frequently asked questions

Which applications is CYP2C9 Polyclonal Antibody (bs-20243R) validated for, and at what dilution?

bs-20243R is validated for ELISA (ELISA=1:5000-10000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500); ICC/IF (ICC/IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CYP2C9 Polyclonal Antibody (bs-20243R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-20243R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CYP2C9 Polyclonal Antibody (bs-20243R)?

KLH conjugated synthetic peptide derived from human CYP2C9

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