Activated Notch1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20252R-BF594 |
| Product Name | Activated Notch1 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Notch 1 intracellular domain. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4851 |
| Swiss Prot | P46531 |
| Subcellular location | Nucleus |
| Synonyms | hN1; AOS5; TAN1; AOVD1; Neurogenic locus notch homolog protein 1; Notch 1; Translocation-associated notch protein TAN-1; NOTCH1 |
| Background | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Activated Notch1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-20252R-BF594) validated for, and at what dilution?
bs-20252R-BF594 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Activated Notch1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-20252R-BF594) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Rabbit, GuineaPig.
How should bs-20252R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Activated Notch1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-20252R-BF594)?
KLH conjugated synthetic peptide derived from human Notch 1 intracellular domain.
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-20252R-BF594)?
AbBy Fluor-594 on bs-20252R-BF594: excitation / emission 590nm/617nm.