Help

HARS Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-20281R-PE-Cy5
Product Name HARS Polyclonal Antibody, PE-Cy5 Conjugated
Applications
WB
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HARS
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3035
Swiss Prot P12081
Subcellular location Cytoplasm
Synonyms EC 6.1.1.21; FLJ20491; HisRS; Jo-1; histidine translase; Histidine tRNA ligase; Histidyl tRNA synthetase; HRS; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds; SYHC_HUMAN.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is HARS Polyclonal Antibody, PE-Cy5 Conjugated (bs-20281R-PE-Cy5) validated for, and at what dilution?

bs-20281R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HARS Polyclonal Antibody, PE-Cy5 Conjugated (bs-20281R-PE-Cy5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Horse, Rabbit.

How should bs-20281R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HARS Polyclonal Antibody, PE-Cy5 Conjugated (bs-20281R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human HARS

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-20281R-PE-Cy5)?

PE-Cy5 on bs-20281R-PE-Cy5: excitation / emission 488nm/670nm.