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TRIM23 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-20285R-PE
Product Name TRIM23 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Sheep, Pig, Horse
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TRIM23
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 373
Swiss Prot P36406
Subcellular location Cytoplasm
Synonyms ADP ribosylation factor domain protein 1; ARF domain protein 1; ARFD1; GTP binding protein ARD 1; TRI23_HUMAN; N-acetyltransferase ARD1 human homolog of; N-acetyltransferase homolog of S. cerevisiae ARD1; TRIM23; Tripartite motif protein TRIM23.
Background Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is TRIM23 Polyclonal Antibody, PE Conjugated (bs-20285R-PE) validated for, and at what dilution?

bs-20285R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TRIM23 Polyclonal Antibody, PE Conjugated (bs-20285R-PE) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Sheep, Pig, Horse.

How should bs-20285R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TRIM23 Polyclonal Antibody, PE Conjugated (bs-20285R-PE)?

KLH conjugated synthetic peptide derived from human TRIM23

What are the excitation and emission wavelengths of the PE conjugate (bs-20285R-PE)?

PE on bs-20285R-PE: excitation / emission 496,564nm/578nm.