CD43 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20324R-RBITC |
| Product Name | CD43 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD43 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6693 |
| Swiss Prot | P16150 |
| Subcellular location | Cell membrane |
| Synonyms | LSN; CD43; GALGP; GPL115; Leukosialin; Galactoglycoprotein; Leukocyte sialoglycoprotein; Sialophorin; SPN |
| Background | One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CD43 Polyclonal Antibody, RBITC Conjugated (bs-20324R-RBITC) validated for, and at what dilution?
bs-20324R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD43 Polyclonal Antibody, RBITC Conjugated (bs-20324R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-20324R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD43 Polyclonal Antibody, RBITC Conjugated (bs-20324R-RBITC)?
KLH conjugated synthetic peptide derived from human CD43
What are the excitation and emission wavelengths of the RBITC conjugate (bs-20324R-RBITC)?
RBITC on bs-20324R-RBITC: excitation / emission 540nm/625nm.