E.coli DH-5 Alpha Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2033R-APC |
| Product Name | E.coli DH-5 Alpha Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Bacteria, Others |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | Whole cell protein of Ecoli DH-5 Alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Escherichia coli DH-5 Alpha; E.coli DH5 Alpha; E.coli DH 5 Alpha. |
| Background | This antibody reacts with Escherichia coli HD5a (E.coli HD5a), Escherichia coli is a gram-negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. E. coli DH5_ strain is an engineering strain widely used in biopharmaceuticals. According to the requirements of product quality inspection, it is necessary to detect the residual amount of DH5_ bacterial protein in biological products. This product is an anti-serum obtained by immunizing rabbits with DH5_ bacterial protein as an antigen, and purified by affinity chromatography to obtain antibody IgG, which can be used for ELISA, Western Blot and other experimental tests. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is E.coli DH-5 Alpha Polyclonal Antibody, APC Conjugated (bs-2033R-APC) validated for, and at what dilution?
bs-2033R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does E.coli DH-5 Alpha Polyclonal Antibody, APC Conjugated (bs-2033R-APC) react with?
Tested reactivity: Bacteria, Others.
How should bs-2033R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise E.coli DH-5 Alpha Polyclonal Antibody, APC Conjugated (bs-2033R-APC)?
Whole cell protein of Ecoli DH-5 Alpha
What are the excitation and emission wavelengths of the APC conjugate (bs-2033R-APC)?
APC on bs-2033R-APC: excitation / emission 650nm/660nm.