CHRM4 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20372R-BF647 |
| Product Name | CHRM4 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CHRM4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1132 |
| Swiss Prot | P08173 |
| Subcellular location | Cell membrane |
| Synonyms | mAChR M4; Muscarinic Acetylcholine Receptor M4; Cholinergic receptor muscarinic 4; Chrm 4; CHRM4; HM 4; HM4; M4; Muscarinic acetylcholine receptor M4; ACM4_HUMAN. |
| Background | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CHRM4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-20372R-BF647) validated for, and at what dilution?
bs-20372R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CHRM4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-20372R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-20372R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CHRM4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-20372R-BF647)?
KLH conjugated synthetic peptide derived from human CHRM4
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-20372R-BF647)?
AbBy Fluor-647 on bs-20372R-BF647: excitation / emission 650nm/665nm.