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HIF-1 Alpha Polyclonal Antibody, PE Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-20398R-PE-100uL
Product Name HIF-1 Alpha Polyclonal Antibody, PE Conjugated
Applications
WB
Reactivity Human, Mouse, Rat
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HIF-1 Alpha
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3091
Swiss Prot Q16665
Synonyms ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor-1a; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8.
Background Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.
Application Dilution
WB FCM

Frequently asked questions

Which applications is HIF-1 Alpha Polyclonal Antibody, PE Conjugated (bs-20398R-PE) validated for, and at what dilution?

bs-20398R-PE is validated for FCM.

Which species does HIF-1 Alpha Polyclonal Antibody, PE Conjugated (bs-20398R-PE) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-20398R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HIF-1 Alpha Polyclonal Antibody, PE Conjugated (bs-20398R-PE)?

KLH conjugated synthetic peptide derived from human HIF-1 Alpha

What are the excitation and emission wavelengths of the PE conjugate (bs-20398R-PE)?

PE on bs-20398R-PE: excitation / emission 496,564nm/578nm.