HIF-1 Alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20399R-BF488 |
| Product Name | HIF-1 Alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIF-1 Alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3091 |
| Swiss Prot | Q16665 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HIF-1-alpha; hypoxia-inducible factor 1, alpha subunit isoform 1; Hypoxia-inducible factor 1 alpha; HIF-1 alpha; HIF1 alpha; HIF-1a; HIF-1alpha; ARNT-interacting protein; Member of PAS protein1; Basic-helix-loop-helix-PAS protein MOP1; Hypoxia-inducible factor -1 Alpha. |
| Background | Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HIF-1 Alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20399R-BF488) validated for, and at what dilution?
bs-20399R-BF488 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIF-1 Alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20399R-BF488) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Chicken.
How should bs-20399R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIF-1 Alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20399R-BF488)?
KLH conjugated synthetic peptide derived from human HIF-1 Alpha
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-20399R-BF488)?
AbBy Fluor-488 on bs-20399R-BF488: excitation / emission 499nm/519nm.