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phospho-APG4B (Ser34) Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
Overview
Catalog # bs-20429R-Cy7-100uL
Product Name phospho-APG4B (Ser34) Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human APG4B around the phosphorylation site of Ser34
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23192
Swiss Prot Q9Y4P1
Subcellular location Cytoplasm
Synonyms APG4B (phospho-Ser34); APG4B (phospho Ser34); APG4B (phospho S34); APG4B (p-Ser34); p-APG4B (Ser34); APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); AUT like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353br; ATG4B_HUMAN.
Application Dilution
WB =1:500-2000
IF

Frequently asked questions

Which applications is phospho-APG4B (Ser34) Polyclonal Antibody, Cy7 Conjugated (bs-20429R-Cy7) validated for, and at what dilution?

bs-20429R-Cy7 is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-APG4B (Ser34) Polyclonal Antibody, Cy7 Conjugated (bs-20429R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.

How should bs-20429R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-APG4B (Ser34) Polyclonal Antibody, Cy7 Conjugated (bs-20429R-Cy7)?

KLH conjugated Synthesised phosphopeptide derived from human APG4B around the phosphorylation site of Ser34

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-20429R-Cy7)?

Cy7 on bs-20429R-Cy7: excitation / emission 743nm/767nm.