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phospho-APG4B (Ser34) Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
Overview
Catalog # bs-20429R-FITC-100uL
Product Name phospho-APG4B (Ser34) Polyclonal Antibody, FITC Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human APG4B around the phosphorylation site of Ser34
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23192
Swiss Prot Q9Y4P1
Subcellular location Cytoplasm
Synonyms APG4B (phospho-Ser34); APG4B (phospho Ser34); APG4B (phospho S34); APG4B (p-Ser34); p-APG4B (Ser34); APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); AUT like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353br; ATG4B_HUMAN.
Application Dilution
WB =1:500-2000
IF

Frequently asked questions

Which applications is phospho-APG4B (Ser34) Polyclonal Antibody, FITC Conjugated (bs-20429R-FITC) validated for, and at what dilution?

bs-20429R-FITC is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-APG4B (Ser34) Polyclonal Antibody, FITC Conjugated (bs-20429R-FITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.

How should bs-20429R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-APG4B (Ser34) Polyclonal Antibody, FITC Conjugated (bs-20429R-FITC)?

KLH conjugated Synthesised phosphopeptide derived from human APG4B around the phosphorylation site of Ser34

What are the excitation and emission wavelengths of the FITC conjugate (bs-20429R-FITC)?

FITC on bs-20429R-FITC: excitation / emission 494nm/518nm.