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Semaphorin 5A Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-20430R-Cy5-100uL
Product Name Semaphorin 5A Polyclonal Antibody, Cy5 Conjugated
Applications
WB
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Semaphorin 5A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9037
Swiss Prot Q13591
Subcellular location Cell membrane
Synonyms Semaphorin 5A; Semaphorin-5A; Semaphorin5A; SEMA5A; SEMAF; Semaphorin F; SEM5A_HUMAN.
Background This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Semaphorin 5A Polyclonal Antibody, Cy5 Conjugated (bs-20430R-Cy5) validated for, and at what dilution?

bs-20430R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Semaphorin 5A Polyclonal Antibody, Cy5 Conjugated (bs-20430R-Cy5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit.

How should bs-20430R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Semaphorin 5A Polyclonal Antibody, Cy5 Conjugated (bs-20430R-Cy5)?

KLH conjugated synthetic peptide derived from human Semaphorin 5A

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-20430R-Cy5)?

Cy5 on bs-20430R-Cy5: excitation / emission 625,650nm/670nm.