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SIP1 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-20484r-fitc-100uL
Product Name SIP1 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SIP1
Immunogen Range 1061-1160/1214
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9839
Swiss Prot O60315
Synonyms Smad Interacting Protein 1 SIP 1; SIP1 SIP-1; Smad-interacting protein 1; SMADIP 1; SMADIP1; ZEB 2; ZEB2; ZEB2_HUMAN; Zfhx1b; ZFHX1B protein; Zfx1b; Zinc finger E box binding protein 2; Zinc finger E-box-binding homeobox 2; Zinc finger homeobox 1b; zinc finger homeobox protein 1; Zinc finger homeobox protein 1b.
Background Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SIP1 Polyclonal Antibody, FITC Conjugated (bs-20484R-FITC) validated for, and at what dilution?

bs-20484R-FITC is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SIP1 Polyclonal Antibody, FITC Conjugated (bs-20484R-FITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-20484R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SIP1 Polyclonal Antibody, FITC Conjugated (bs-20484R-FITC)?

KLH conjugated synthetic peptide derived from human SIP1 Immunogen range: 1061-1160/1214.

What are the excitation and emission wavelengths of the FITC conjugate (bs-20484R-FITC)?

FITC on bs-20484R-FITC: excitation / emission 494nm/518nm.