SIP1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20485R-RBITC-100uL |
| Product Name | SIP1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SIP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9839 |
| Swiss Prot | O60315 |
| Synonyms | Smad Interacting Protein 1; SIP 1; SIP1; SIP-1; Smad-interacting protein 1; SMADIP 1; SMADIP1; ZEB 2; ZEB2; ZEB2_HUMAN; Zfhx1b; ZFHX1B protein; Zfx1b; Zinc finger E box binding protein 2; Zinc finger E-box-binding homeobox 2; Zinc finger homeobox 1b; zinc finger homeobox protein 1; Zinc finger homeobox protein 1b. |
| Background | Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SIP1 Polyclonal Antibody, RBITC Conjugated (bs-20485R-RBITC) validated for, and at what dilution?
bs-20485R-RBITC is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SIP1 Polyclonal Antibody, RBITC Conjugated (bs-20485R-RBITC) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse.
How should bs-20485R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SIP1 Polyclonal Antibody, RBITC Conjugated (bs-20485R-RBITC)?
KLH conjugated synthetic peptide derived from human SIP1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-20485R-RBITC)?
RBITC on bs-20485R-RBITC: excitation / emission 540nm/625nm.