PODXL Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20488R-FITC-100uL |
| Product Name | PODXL Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse PODXL |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27205 |
| Swiss Prot | Q9R0M4 |
| Subcellular location | Cell membrane |
| Synonyms | Podocalyxin; Podxl; Podocalyxin-like protein 1; PC; PCLP-1; Pclp1 |
| Background | Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is PODXL Polyclonal Antibody, FITC Conjugated (bs-20488R-FITC) validated for, and at what dilution?
bs-20488R-FITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PODXL Polyclonal Antibody, FITC Conjugated (bs-20488R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-20488R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PODXL Polyclonal Antibody, FITC Conjugated (bs-20488R-FITC)?
KLH conjugated synthetic peptide derived from mouse PODXL
What are the excitation and emission wavelengths of the FITC conjugate (bs-20488R-FITC)?
FITC on bs-20488R-FITC: excitation / emission 494nm/518nm.