OLIG3 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20543R-Cy7 |
| Product Name | OLIG3 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human OLIG3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 167826 |
| Swiss Prot | Q7RTU3 |
| Subcellular location | Nucleus |
| Synonyms | Basic helix loop helix domain containing class B protein 7; Bhlhb7; bHLHe20; Class B basic helix loop helix protein 7; Class B basic helix-loop-helix protein 7; Class E basic helix loop helix protein 20; Class E basic helix-loop-helix protein 20; Olig3; OLIG3_HUMAN; Oligo3; Oligodendrocyte specific bHLH transcription factor 3; Oligodendrocyte transcription factor 3 |
| Background | May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is OLIG3 Polyclonal Antibody, Cy7 Conjugated (bs-20543R-Cy7) validated for, and at what dilution?
bs-20543R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does OLIG3 Polyclonal Antibody, Cy7 Conjugated (bs-20543R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-20543R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise OLIG3 Polyclonal Antibody, Cy7 Conjugated (bs-20543R-Cy7)?
KLH conjugated synthetic peptide derived from human OLIG3
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-20543R-Cy7)?
Cy7 on bs-20543R-Cy7: excitation / emission 743nm/767nm.