NCX3 Polyclonal Antibody

Formalin-fixed and rat brain tissue labeled with Anti-NCX3 Polyclonal Antibody (bs-2057R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A549 cell lysates probed with NCX3 Polyclonal Antibody, Unconjugated (bs-2057R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A549 cell lysates probed with NCX3 Polyclonal Antibody, Unconjugated (bs-2057R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat
Bovine
Dog
Pig
Chicken

Overview
Catalog #	bs-2057r
Product Name	NCX3 Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Human, Mouse, Rat, Bovine, Dog, Pig, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NCX3
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6547
Subcellular location	Cytoplasm
Synonyms	AW742262; MGC90626; Na+/Ca2+-exchange protein 3; Na+/Ca2+ exchange protein 3; Na+/Ca2+ exchanger 3; NAC3_HUMAN; NCX3; SCL8A3; Sodium calcium exchanger form 3; Sodium/calcium exchanger 3; Sodium/calcium exchanger SLC8A3; Solute carrier family 8 sodium calcium exchanger member 3.
Background	SLC8A3 is a member of the sodium/calcium exchanger integral membrane protein family. Three mammalian isoforms in family 8 have been identified. Na+/Ca2+ exchange proteins are involved in maintaining Ca2+ homeostasis in a wide variety of cell types. The protein is regulated by intracellular calcium ions and is found in both the plasma membrane and intracellular organellar membranes, where exchange of Na+ for Ca2+ occurs in an electrogenic manner.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200