BNC1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (BNC1) Polyclonal Antibody, Unconjugated (bs-20583R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (BNC1) Polyclonal Antibody, Unconjugated (bs-20583R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-20583r
Product Name	BNC1 Polyclonal Antibody
Applications	IHC-P IHC-F IF ICC/IF
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BNC1
Immunogen Range	1-100/944
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	646
Swiss Prot	Q01954
Subcellular location	Cytoplasm, Nucleus
Synonyms	basonuclin; basonuclin 1; basonuclin1; basonuclin-1; BNC; BNC1_HUMAN; BSN1; HsT19447; zinc finger protein basonuclin; Zinc finger protein basonuclin-1.
Background	The zinc finger protein, basonuclin, is a putative rDNA transcription factor with highly restricted tissue distribution. Basonuclin is abundantly expressed in keratinocytes of the basal layer of the epidermis, the outer sheath of hair follicles and in the germ cells of the testis and ovary. Although basonuclin is associated with chromatin throughout the cell cycle, including mitosis, it disappears when cells become post-mitotic. In the epidermis, basonuclin, which is mainly localized to the cytoplasm, translocates to basal cell nuclei during different stages of keratinocyte growth. Basonuclin may enhance rRNA synthesis by elevating transcription from an rDNA promoter and inhibiting RNA polymerase I transcription through its zinc finger domain. Therefore, basonuclin may be a cell-type-specific transcription factor for rDNA transcription.
Application Dilution
IHC-P	=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500
ICC/IF	ICC/IF=1:50