BNC1 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20583r |
| Product Name | BNC1 Polyclonal Antibody |
| Applications |
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BNC1 |
| Immunogen Range | 1-100/944 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 646 |
| Swiss Prot | Q01954 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | basonuclin; basonuclin 1; basonuclin1; basonuclin-1; BNC; BNC1_HUMAN; BSN1; HsT19447; zinc finger protein basonuclin; Zinc finger protein basonuclin-1. |
| Background | The zinc finger protein, basonuclin, is a putative rDNA transcription factor with highly restricted tissue distribution. Basonuclin is abundantly expressed in keratinocytes of the basal layer of the epidermis, the outer sheath of hair follicles and in the germ cells of the testis and ovary. Although basonuclin is associated with chromatin throughout the cell cycle, including mitosis, it disappears when cells become post-mitotic. In the epidermis, basonuclin, which is mainly localized to the cytoplasm, translocates to basal cell nuclei during different stages of keratinocyte growth. Basonuclin may enhance rRNA synthesis by elevating transcription from an rDNA promoter and inhibiting RNA polymerase I transcription through its zinc finger domain. Therefore, basonuclin may be a cell-type-specific transcription factor for rDNA transcription. |
| Application Dilution | |
| IHC-P | =1:100-500 |
| IHC-F | IHC-F=1:100-500 |
| IF | IF=1:100-500 |
| ICC/IF | ICC/IF=1:50 |
Frequently asked questions
Which applications is BNC1 Polyclonal Antibody (bs-20583R) validated for, and at what dilution?
bs-20583R is validated for IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500); ICC/IF (ICC/IF=1:50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BNC1 Polyclonal Antibody (bs-20583R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-20583R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BNC1 Polyclonal Antibody (bs-20583R)?
KLH conjugated synthetic peptide derived from human BNC1 Immunogen range: 1-100/944.