DDIT3 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20669R-HRP-100uL |
| Product Name | DDIT3 Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GADD153/DDIT3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7039 |
| Swiss Prot | P35638 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | EGF like TGF; ETGF; TFGA; TGF A; TGF type 1; TGFA; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1; TGFA_HUMAN. |
| Background | TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar (By similarity). Inhibitor of acid secretion. Inhibitor of aminopyrine uptake in parietal cells (in vitro). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is DDIT3 Polyclonal Antibody, HRP Conjugated (bs-20669R-HRP) validated for, and at what dilution?
bs-20669R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DDIT3 Polyclonal Antibody, HRP Conjugated (bs-20669R-HRP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-20669R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DDIT3 Polyclonal Antibody, HRP Conjugated (bs-20669R-HRP)?
KLH conjugated synthetic peptide derived from human GADD153/DDIT3