DDIT3 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20669R-PerCP-100uL |
| Product Name | DDIT3 Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GADD153/DDIT3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7039 |
| Swiss Prot | P35638 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | EGF like TGF; ETGF; TFGA; TGF A; TGF type 1; TGFA; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1; TGFA_HUMAN. |
| Background | TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar (By similarity). Inhibitor of acid secretion. Inhibitor of aminopyrine uptake in parietal cells (in vitro). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DDIT3 Polyclonal Antibody, PerCP Conjugated (bs-20669R-PerCP) validated for, and at what dilution?
bs-20669R-PerCP is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DDIT3 Polyclonal Antibody, PerCP Conjugated (bs-20669R-PerCP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-20669R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DDIT3 Polyclonal Antibody, PerCP Conjugated (bs-20669R-PerCP)?
KLH conjugated synthetic peptide derived from human GADD153/DDIT3
What are the excitation and emission wavelengths of the PerCP conjugate (bs-20669R-PerCP)?
PerCP on bs-20669R-PerCP: excitation / emission 482nm/677nm.