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ROR alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-20687R-BF488-100uL
Product Name ROR alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ROR alpha
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6095
Swiss Prot P35398
Subcellular location Nucleus
Synonyms NR1F1; Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; Retinoid-related orphan receptor-alpha; Rora; RORA_HUMAN; RZR-ALPHA; RZRA.
Background The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is ROR alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20687R-BF488) validated for, and at what dilution?

bs-20687R-BF488 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ROR alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20687R-BF488) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken.

How should bs-20687R-BF488 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ROR alpha Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20687R-BF488)?

KLH conjugated synthetic peptide derived from human ROR alpha

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-20687R-BF488)?

AbBy Fluor-488 on bs-20687R-BF488: excitation / emission 499nm/519nm.