Galectin 3 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20700R-APC |
| Product Name | Galectin 3 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Galectin 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1385 |
| Swiss Prot | P17931 |
| Subcellular location | Cytoplasm, Nucleus, Secreted |
| Synonyms | Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB1; CREB 1; CREB1; MGC9284; Transactivator protein; CREB; CREB1_HUMAN. |
| Background | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Galectin 3 Polyclonal Antibody, APC Conjugated (bs-20700R-APC) validated for, and at what dilution?
bs-20700R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Galectin 3 Polyclonal Antibody, APC Conjugated (bs-20700R-APC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog.
How should bs-20700R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Galectin 3 Polyclonal Antibody, APC Conjugated (bs-20700R-APC)?
KLH conjugated synthetic peptide derived from human Galectin 3
What are the excitation and emission wavelengths of the APC conjugate (bs-20700R-APC)?
APC on bs-20700R-APC: excitation / emission 650nm/660nm.