NOS-2/iNOS Polyclonal Antibody

$Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI$

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI

$Formalin-fixed and paraffin embedded rat lung labeled with Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat lung labeled with Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded human kidney labeled with Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human kidney labeled with Anti-NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat
Dog

Overview
Catalog #	bs-2072R
Product Name	NOS-2/iNOS Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat, Dog
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse NOS-2
Immunogen Range	1-50/1144
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID	18126
Swiss Prot	P29477
Synonyms	iNOS; Nos-2; Nos2a; i-NOS; NOS-II; Nitric oxide synthase, inducible; Inducible NO synthase; Inducible NOS; Macrophage NOS; MAC-NOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2; Nos2; Inosl
Background	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Application Dilution
WB	1:300-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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