iNOS Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2072R-APC-Cy7-100uL |
| Product Name | iNOS Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Mouse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse NOS-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 18126 |
| Swiss Prot | P29477 |
| Subcellular location | Cytoplasm |
| Synonyms | iNOS; Nos-2; Nos2a; i-NOS; NOS-II; Nitric oxide synthase, inducible; Inducible NO synthase; Inducible NOS; Macrophage NOS; MAC-NOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2; Nos2; Inosl |
| Background | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is iNOS Polyclonal Antibody, APC-Cy7 Conjugated (bs-2072R-APC-Cy7) validated for, and at what dilution?
bs-2072R-APC-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does iNOS Polyclonal Antibody, APC-Cy7 Conjugated (bs-2072R-APC-Cy7) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rabbit.
How should bs-2072R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise iNOS Polyclonal Antibody, APC-Cy7 Conjugated (bs-2072R-APC-Cy7)?
KLH conjugated synthetic peptide derived from mouse NOS-2
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-2072R-APC-Cy7)?
APC-Cy7 on bs-2072R-APC-Cy7: excitation / emission 650nm/780nm.