IRS-2 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20748R-APC-Cy5.5-100uL |
| Product Name | IRS-2 Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Horse |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IRS-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8660 |
| Swiss Prot | Q9Y4H2 |
| Subcellular location | Cytoplasm |
| Synonyms | Insulin Receptor Substrate 2; IRS 2; IRS-2; IRS2; IRS2_HUMAN. |
| Background | This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IRS-2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-20748R-APC-CY5-5) validated for, and at what dilution?
BS-20748R-APC-CY5-5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IRS-2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-20748R-APC-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Horse.
How should BS-20748R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IRS-2 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-20748R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human IRS-2
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-20748R-APC-CY5-5)?
APC-Cy5.5 on BS-20748R-APC-CY5-5: excitation / emission 650nm/694nm.